Influenza A NP Antibody PA5-32242 from Thermo Fisher Scientific

Product Specs
ItemInfluenza A NP Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPA5-32242
ApplicationsWB, ELISA, ICC, IHC-p
ReactivityVirus (Vi)
Concentration0.15 mg/mL
ImmunogenRecombinant protein fragment of Influenza A virus NP (A/WSN/1933(H1N1))
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles
HostRabbit
IsotypeIgG
TargetInfluenza A NP
ClonalityPolyclonal
FormatPBS with 20% glycerol and 0.025% ProClin 300; pH 7
Conjugate/TagUnconjugated
Quantity100 µL

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) ABTB1 facilitates the replication of influenza A virus by counteracting TRIM4-mediated degradation of viral NP proteinEmerging Microbes & InfectionsDecember 8, 2023Wenjun Shi, Zhibo Shan, Li Jiang, Guangwen Wang, Xuyuan Wang, Yu Chang, Yuzhen Hu, Bo Wang, Qibing Li, Yihan Wang, et al.primary antibodies were purchased from commercial sources: rabbit anti-NP polyclonal antibody (pAb) (PA5-32242, Invitrogen, Carlsbad, CA, USA), rabbit anti-M1 pAb (GTX125928, GeneTex, Irvine, CA, USA), rabbit anti-HA

(2) NRF2 activators inhibit influenza A virus replication by interfering with nucleo-cytoplasmic export of viral RNPs in an NRF2-independent mannerPLOS PathogensJuly 17, 2023Fakhar H. Waqas, Mahmoud Shehata, Walid A. M. Elgaher, Antoine Lacour, Naziia Kurmasheva, Fabio Begnini, Anders E. Kiib, Julia Dahlmann, Chutao Chen, Andreas Pavlou, et al.Collégien, France). The following primary antibodies were used: influenza A virus nucleoprotein (NP, PA5-32242, 1:3000, ThermoFisher Scientific), XPO1 (46249S, 1:1000, Cell signaling technology), β-actin (ab49900,

(3) Analyzing immune responses to varied mRNA and protein vaccine sequencesNPJ vaccinesJune 5, 2023Park HJ, Bang YJ, Kwon SP, Kwak W, Park SI, Roh G, Bae SH, Kim JY, Kwak HW, Kim Y, et al.Cat: 14-0081-82, 53-6.7, Invitrogen), and Influenza A NP polyclonal antibody (1:200 dilution, Cat: PA5-32242, Invitrogen)—overnight at 4 °C. This was followed by incubation with biotinylated universal secondary

(4) Adenoviral-vectored epigraph vaccine elicits robust, durable, and protective immunity against H3 influenza A virus in swineFrontiers in ImmunologyMay 15, 2023Erika Petro-Turnquist, Matthew Pekarek, Nicholas Jeanjaquet, Cedric Wooledge, David Steffen, Hiep Vu, Eric A. Weaverconserved nucleoprotein (NP) viral antigen using a rabbit anti-Influenza A virus NP antibody (Cat. No. PA5-32242; Invitrogen). The formalin-fixed tissues were processed routinely for histologic examination after 72

(5) FDA-approved Abl/EGFR/PDGFR kinase inhibitors show potent efficacy against pandemic and seasonal influenza A virus infections of human lung explantsiScienceApril 21, 2023Robert Meineke, Sonja Stelz, Maximilian Busch, Christopher Werlein, Mark Kühnel, Danny Jonigk, Guus F. Rimmelzwaan, Husni ElbaheshSignaling), phos-Stat3 (Tyr705) (D3A7) rabbit mAb (1:1000) (Cell Signaling), Influenza A virus NP Antibody (PA5-32242) rabbit pAb (1:20,000) (ThermoScientific). Beta-Actin (BA3R) mAb (1:5000) was used as a loading control.

(6) Generalized Modules for Membrane Antigens (GMMA), an outer membrane vesicle-based vaccine platform, for efficient viral antigen deliveryJournal of extracellular vesiclesNovember 1, 2022Hu K, Palmieri E, Samnuan K, Ricchetti B, Oldrini D, McKay PF, Wu G, Thorne L, Fooks AR, McElhinney LM, et al.blocked with 5% non‐fat milk in PBST, and then sequentially incubated with rabbit anti‐NP antibody (PA5‐32242; Thermo Fisher Scientific) and mouse anti‐rabbit IgG‐HRP (sc2357; Santa Cruz). After extensive washes,

(7) Optimizing the Live Attenuated Influenza A Vaccine Backbone for High-Risk Patient GroupsJournal of VirologyOctober 26, 2022JoÃ£o P. P. L. Bonifacio, Nathalia Williams, Laure Garnier, Stephanie Hugues, Mirco Schmolke, Beryl Mazel-SanchezA8592), rabbit anti-XBP1 (ThermoFisher number PA5-27650), rabbit anti-influenza NP (ThermoFisher number PA5-32242), rabbit anti-influenza NS1 (1-93) (kind gift from A. Garcìa-Sastre) and goat anti-rabbit IgG HRP (Sigma-Aldrich

(8) Real-time tracking of bioluminescent influenza A virus infection in miceScientific reportsFebruary 24, 2022Kim JH, Bryant H, Fiedler E, Cao T, Rayner JO.polyclonal rabbit anti-PB2 (PA5-32220, Invitrogen), rabbit anti-PA (PA5-32223, Invitrogen), rabbit anti-NP (PA5-32242, Invitrogen), monoclonal mouse anti-NanoLuc luciferase (MAB10026, R&D systems), or mouse anti-β-actin

(9) Non-classical autophagy activation pathways are essential for production of infectious Influenza A virus in vitroMolecular MicrobiologyFebruary 1, 2022Tisza A. S. Bell, Nileena Velappan, Cheryl D. Gleasner, Gang Xie, Shawn R. Starkenburg, Geoffrey Waldo, Shounak Banerjee, Sofiya N. Micheva‐Viteva(Szretter et al., 2006), and immunoplaque analysis was performed with anti‐NP antibody (ThermoFisher, PA5‐32242) and alkaline phosphatase‐conjugated anti‐rabbit antibody. To study the dynamics of viral RNA replication,

(10) The IFN-inducible GTPase IRGB10 regulates viral replication and inflammasome activation during influenza A virus infection in miceEuropean journal of immunologyFebruary 1, 2022Christgen S, Place DE, Zheng M, Briard B, Yamamoto M, Kanneganti TD.IAV NS1 (1:1000 dilution, Santa Cruz, sc130568); IAV NP (1:1000 dilution, Thermo Fisher Scientific, PA5–32242); pERK (1:1000 dilution, CST, 9101); ERK (1:1000 dilution, CST, 9102); pSTAT1 (1:1000 dilution, CST,

(11) T resident helper cells promote humoral responses in the lungScience ImmunologyJanuary 8, 2021Nivedya Swarnalekha, David Schreiner, Ludivine C. Litzler, Saadia Iftikhar, Daniel Kirchmeier, Marco Künzli, Young Min Son, Jie Sun, Etori Aguiar Moreira, Carolyn G. Kingwere visualized 72 hours after infection using a primary anti-NP antibody (Thermo Fisher Scientific, PA5-32242) diluted 1:2000 in 1% BSA and a secondary fluorescent antibody (Thermo Fisher Scientific, A-11034) diluted

(12) DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon responseThe Journal of biological chemistryJanuary 1, 2021Kesavardhana S, Samir P, Zheng M, Malireddi RKS, Karki R, Sharma BR, Place DE, Briard B, Vogel P, Kanneganti TD.(Bethyl Laboratories, A300-474A), anti-NS1 (Santa Cruz Biotechnology, SC-130568), anti-NP (Thermo Fisher Scientific, PA5-32242), anti-M1 (Abcam, ab20910), anti-G3BP1 (Proteintech, 27299-I-AP), anti–P-IRF3 (#37829S, CST), anti-IRF1

(13) Influenza A viruses limit NLRP3-NEK7-complex formation and pyroptosis in human macrophagesEMBO reportsDecember 3, 2020Boal-Carvalho I, Mazel-Sanchez B, Silva F, Garnier L, Yildiz S, Bonifacio JP, Niu C, Williams N, Francois P, Schwerk N, et al.

(14) Thapsigargin at Non-Cytotoxic Levels Induces a Potent Host Antiviral Response that Blocks Influenza A Virus ReplicationVirusesSeptember 27, 2020Goulding LV, Yang J, Jiang Z, Zhang H, Lea D, Emes RD, Dottorini T, Pu J, Liu J, Chang KC.at 1:500 dilution (Abcam, ab20910), rabbit antiviral NP at 1:500 dilution (Thermo Fisher Scientific, PA5-32242) and mouse anti-β-actin at 1:10,000 (Sigma-Aldrich, A5316), and appropriate species-specific secondary

(15) Structure and Sequence Determinants Governing the Interactions of RNAs with Influenza A Virus Non-Structural Protein NS1VirusesAugust 27, 2020Wacquiez A, Coste F, Kut E, Gaudon V, Trapp S, Castaing B, Marc D.separation and immunoblot detection using the NS1- and NP-specific polyclonal rabbit antisera (anti-NP PA5-32242, Thermo Fischer Scientific, Waltham, MA, USA) and the ECL-system detection (Advansta, San Jose, CA,

(16) Enhancing Neuraminidase Immunogenicity of Influenza A Viruses by Rewiring RNA Packaging SignalsJournal of virologyJuly 30, 2020Zheng A, Sun W, Xiong X, Freyn AW, Peukes J, Strohmeier S, Nachbagauer R, Briggs JAG, Krammer F, Palese P.4A5 (43) (1 ␮g/ml), rabbit anti-H1 (1:3,000, PA5-34929; Thermo Fisher), and rabbit anti-NP (1:3,000, PA5-32242; Invitrogen). For detection of HK14 proteins, the following antibodies were used: mouse anti-H3 monoclonal

(17) The Pyrimidine Analog FNC Potently Inhibits the Replication of Multiple EnterovirusesJournal of virologyApril 16, 2020Xu N, Yang J, Zheng B, Zhang Y, Cao Y, Huan C, Wang S, Chang J, Zhang W.polyclonal goat anti-RSV antibody (AB1128, diluted 1:250; Millipore), polyclonal rabbit anti-NP antibody (PA5-32242, diluted 1:2,500; Thermo Fisher), anti-tubulin monoclonal antibody (MAb; ab11323, diluted 1:1,000; Abcam,

(18) High Levels of miR-483-3p Are Present in Serum Exosomes Upon Infection of Mice With Highly Pathogenic Avian Influenza VirusFrontiers in MicrobiologyFebruary 11, 2020Tadashi Maemura, Satoshi Fukuyama, Yoshihiro Kawaokatemperature. Then, the cells were incubated with a rabbit polyclonal anti-influenza A NP antibody (PA5-32242, Thermo Fisher Scientific) for 1 h at room temperature. Goat anti-rabbit IgG (H + L) secondary antibody,

(19) Testosterone Protects Against Severe Influenza by Reducing the Pro-Inflammatory Cytokine Response in the Murine LungFrontiers in immunologyJanuary 1, 2020Tuku B, Stanelle-Bertram S, Sellau J, Beck S, Bai T, Kouassi NM, Preuß A, Hoenow S, Renné T, Lotter H, et al.Formalin-fixed paraffin-embedded lung thin sections were stained with hematoxylin a rabbit anti-NP antibody (Thermo Fisher, PA5-32242) and a biotin-conjugated-rabbit secondary antibody (Jackson ImmunoResearch, 711-066-152) for immunohistochemical

(20) ANP32B Deficiency Protects Mice From Lethal Influenza A Virus Challenge by Dampening the Host Immune ResponseFrontiers in immunologyJanuary 1, 2020Beck S, Zickler M, Pinho Dos Reis V, Günther T, Grundhoff A, Reilly PT, Mak TW, Stanelle-Bertram S, Gabriel G.immunohistochemically (IHC) using a rabbit anti-influenza nucleoprotein antibody (1:10,000; Life Technologies, #PA5-32242). As a secondary antibody for IHC, a biotin-conjugated anti-rabbit IgG antibody (1:200; Jackson Immuno

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Influenza A NP Antibody

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Influenza A NP Antibody from Thermo Fisher Scientific

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